Biochemical which is used by the biochemists

Biochemical technique is the tool or method which is used
by the biochemists in their research, laboratory and daily bases.

They gave an idea to
understand about the structure of gene, gene expression, bimolecules and their
functions such as protein, carbohydrates, lipids, nucleic acid and also help to
diagnose the diseases.

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These  techniques are mainly perfusion, cell
fractionation or centrifugation, homogenization, purification, chromatography, microscopy,
crystallography, enzyme or protein assay, PCR, isotropic tracer techniques,
spectrophotometry, colorimetry, electrophoresis, ultra centrifugation,
radioimmuno assay, ELISA and biochemical tests.

Biochemical techniques that used mostly:

Ø Microscopy

Cells are too small to seen by naked eye, in consequence,
the microscope is an instrument which is used to analyze the cell, its
organelles, and even its molecules can also be seen under the Microscope.
The Microscope is based on the technique which is called as Microscopy,
which portrayed the principle on which microscope work.

There are number of
microscopic techniques used to magnify the objects which are included in below

Optical Or Light Microscopy

Electron Microscopy


Ø Colorimetry

It is the technique which is
based on colorimeter, can determined the concentration of unknown sample by
measuring the absorbance of specific wavelength of light.


Ø Chromatography

It is the technique used to
separate small quantities of complex mixtures and its components which are
depends upon solid(or stationary phase) and liquid, gas (or mobile phase).

It is of many types few are
as follow:


Gel filteration chromatography


High performance
liquid chromatography


Column chromatography


Ø Electrophoresis

is the technique based on the movement of electrical charges under the
influence of electric current through an electrolyte for the separation of proteins,
nucleic acid and peptides.

Electrophoresis is of many

electrophoresis (agarose, PAGE)


acetate membrane electrophoresis


Ø Centrifugation

is the technique of separation of different components of a mixture according to
their molecular weight, size and density by spinning or rotating sample on high
speed that component becomes settled down and supernatant obtained through
centrifugal force. It is of many types:

Differential centrifugation

Rate zonal density
gradient centrifugation

Isopycnic density
gradient centrifugation

Ultra centrifugation


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